Enzyme composition of buffalo seminal plasma.

The preservation quality of buffalo semen is poor. The possibility that the cause is inherent in the enzyme system of the spermatozoa and seminal plasma has not so far been systematically studied. There are a few reports on acid and alkaline phosphatase (Eapen, Shrivastava & RazaNasir, 1946; Roy, Pandey & Rawat, 1960; Sengupta, Misra & Roy, 1963; Misra, Singh & Tomar, 1969), but no reports appear to be available on the normal activity of glutamic\x=req-\ oxaloacetic transaminase (GOT), glutamic-pyruvic transaminase (GPT), lactic dehydrogenase (LDH) and aldolase in buffalo seminal plasma. Most of the enzymes, the activities of which have been estimated in seminal plasma, are also to be found in spermatozoa and there is a considerable possibility of `leakage' of some of the intracellular enzymes, e.g. LDH and GOT. We have attempted to study this problem in connection with the distribution of aldolase (Chauhan & Srivastava, 1973). The present communication deals with the normal values of the enzymes quoted above in buffalo seminal plasma. The semen samples were obtained from six buffalo bulls. The seminal plasma was obtained by centrifugation of semen at 7000 g at 4\s=deg\C for 10 min in a refrigerated centrifuge. The time lapse between collection and assay of samples